A New Chamber for Studying the Behavior of Drosophila
Written by Scott Christley et al. on January 27, 2010 – 8:00 am -Methods available for quickly and objectively quantifying the behavioral phenotypes of the fruit fly, Drosophila melanogaster, lag behind in sophistication the tools developed for manipulating their genotypes. We have developed a simple, easy-to-replicate, general-purpose experimental chamber for studying the ground-based behaviors of fruit flies. The major innovative feature of our design is that it restricts flies to a shallow volume of space, forcing all behavioral interactions to take place within a monolayer of individuals. The design lessens the frequency that flies occlude or obscure each other, limits the variability in their appearance, and promotes a greater number of flies to move throughout the center of the chamber, thereby increasing the frequency of their interactions. The new chamber design improves the quality of data collected by digital video and was conceived and designed to complement automated machine vision methodologies for studying behavior. Novel and improved methodologies for better quantifying the complex behavioral phenotypes of Drosophila will facilitate studies related to human disease and fundamental questions of behavioral neuroscience.
Posted in Computer Science | Comments Off
Mapping Change in Large Networks
January 27, 2010 – 8:00 amChange is a fundamental ingredient of interaction patterns in biology, ...
Comments OffAnalysis of Compound Synergy in High-Throughput Cellular Screens by Population-Based Lifetime Modeling
January 27, 2010 – 8:00 amDespite the successful introduction of potent anti-cancer therapeutics, most of ...
Comments OffParameter Identifiability and Redundancy: Theoretical Considerations
January 27, 2010 – 8:00 amBackground Models for complex biological systems may involve a large number ...
Comments OffGenome-Wide Analysis of Binding Sites and Direct Target Genes of the Orphan Nuclear Receptor NR2F1/COUP-TFI
January 27, 2010 – 8:00 amBackground Identification of bona fide direct nuclear receptor gene targets has ...
Comments OffA Computer Simulation of Progesterone and Cox2 Inhibitor Treatment for Preterm Labor
January 27, 2010 – 8:00 amBackground Sufficient information from in vitro and in vivo studies has ...
Comments OffA Novel Alignment-Free Method for Comparing Transcription Factor Binding Site Motifs
January 20, 2010 – 8:00 amBackground Transcription factor binding site (TFBS) motifs can be accurately represented ...
Comments OffMolecular Signatures of Quiescent, Mobilized and Leukemia-Initiating Hematopoietic Stem Cells
January 20, 2010 – 8:00 amHematopoietic stem cells (HSC) are rare, multipotent cells capable of ...
Comments OffInsertion and Deletion Processes in Recent Human History
January 19, 2010 – 8:00 amBackground Although insertions and deletions (indels) account for a sizable portion ...
Comments OffStructural Analysis to Determine the Core of Hypoxia Response Network
January 19, 2010 – 8:00 amThe advent of sophisticated molecular biology techniques allows to deduce ...
Comments Off