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This guide contains information about only the Omnibook XE3, technology code GF. This manual and any examples contained herein are provided “as is” and. HP OmniBook xe3-gc manuals. 77 manuals in 21 languages available for free view and download. Office Manuals and free pdf instructions. Find the office and computer equipment manual you need at ManualsOnline.
Hewlett-Packard Company makes no warranty of any kind with regard to this manual, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose. Transfer of Rights in Software. Customer may transfer rights in the software to a third party only as part of the transfer of all rights and only if Customer obtains the prior agreement of the third party to be bound by the terms of this License Agreement.
Contents Introducing Your Computer To safeguard your data To use digitally signed drivers in Windows Specifications and Regulatory Information Your HP notebook PC comes with preinstalled software, and various models are configured with different combinations of software.
This pictorial inttroduction shows you how to get the computer up and Quick Start sheet running quickly. It shows how to set up and operate your computer and where to go for help if you run into trouble. Multimedia buttons The multimedia buttons are on the front of the computer, to the right of the status display. Introducing Your Computer Identifying Parts of the Notebook Status panel The status panel on the front of the computer provides information about CD playback status, system power management, system date and time, and battery status.
Messages are displayed on the 8-character LCD display, and you can switch from one kind of message to another by pressing the mode button to the left of the display.
Power switch and standby button There are two controls above the keyboard that affect power in the Manuak notebook PC. The switch at the top left turns computer power on omnbook off, and the large blue button in the top center puts the system in standby mode—or, if it is already in standby mode, returns it to If the battery has been removed, you can install it by inserting it into the battery bay on the bottom of your computer. Insert the back edge first, as shown, then press down the front edge until the battery latch clicks shut.
Using an unauthorized AC adapter can damage the computer and may void your warranty. Refer to the warranty statement for your product.
If you prefer, you can instead use a PC card modem or an external modem to connect a phone line. Plug in the AC adapter and slide the power switch to the left again.
The first omniblok you turn on your computer, a Windows Setup program will automatically run so that you can customize your setup. If you have Windowsyou are not prompted to register, but you can register by modem if you double-click the HP Notebook Registration icon on the desktop or click the Register link in the Windows welcome screen.
See that the phone line is connected and follow the onscreen instructions. By fax If a printer is connected to your computer, you can print a copy of your registration and fax it to HP. If the computer does not shut down, slide and hold the power switch for five seconds, then release it. Using Your Computer Operating the Computer Operating the Computer To use the Fn hot keys The combination of the Fn key plus another key creates a hot key—a shortcut key sequence—for various system controls.
HP OmniBook XE3 Service Manual
Press and hold Fn while pressing the appropriate key. This is the same as clicking the Start button on the taskbar. The Applications key brings up the shortcut menu for the current application.
This is the same menu that appears when you click the right mouse button while pointing at the application. This is a shift key providing access to certain keyboard characters. To temporarily silence the speaker without changing the volume setting, click Mute. With the multimedia buttons When the computer is on, the volume control buttons on the front of the computer govern volume for most audio applications, including the CD player.
When the computer is off, you can use these buttons to adjust the volume for music Omnibolk. Using Your Computer Operating the Computer Move a thumb or finger across the touch pad in the direction you want the pointer to move.
Use the click buttons, which function like the left and right buttons on a mouse, to make your selection. To use the One-Touch buttons The One-Touch buttons on your Manjal notebook allow you to start your e-mail or a Web browser—or any other application—by pressing a single button.
The buttons are configured initially for specific applications, but you can change the configuration to start any applications you want. Click Done, then OK. To format display of application names When you press a One-Touch button on your HP notebook, the name of the application that is starting appears onscreen. You can change the size, color, and font manal the display, as well as the length omnibolk time it appears.
Click the Size arrow. Select the desired size. Click Preview to see a sample message displayed in that size. To change the length of time the display appears 1. You can improve security by—among other methods—using a password, locking the computer with a cable, or setting up virus protection.
To set up password protection You can protect your computer from access by another user when you set up password protection, which is available either through Windows software or mamual the BIOS Check that you have set up a Windows password, as described above. Lmnibook Start, Settings, Control Panel 3. Using Your Computer Securing the Computer To attach a security cable Your computer has a built-in connector that allows you to secure the computer with a cable.
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The Kensington MicroSaver lock system works with this connector and is available at many computer stores. HP docking accessories offer quick, easy connections to these devices. Position the computer so your wrists and hands are in a neutral position. Your wrists should be as straight as possible and should not have to bend sideways or more than 10 degrees up or down.
Your HP notebook computer has a built-in palm rest, which is ideal for this purpose. To take care of your computer Provide adequate ventilation around the computer. Ensure that the laptop is sitting on a flat surface, so that air is able to flow underneath the unit. See that there is adequate clearance to the rear and sides of the computer, so that fan vents are not blocked. Using Your Computer Caring for Your Computer To safeguard your data Do not touch the touch pad or activate any other device that interrupts operation while the system is starting or stopping.
Back up your work regularly. Copy files to floppy disk, tape, or network drives. Use a virus-scanning program, such as the included application VirusScan, for exampleto check the integrity of your files and operating system. To get the most from your batteries Do not leave batteries unused for long periods of time. If you have more than one, rotate them.
If you normally use AC power, make a practice, at least once a week, of using the battery as your power source. Traveling with Your Computer Managing Power Consumption Managing Power Consumption When you are not using your computer, you will want to maximize battery operating time without compromising performance. It is not necessary to turn the computer off in order to reduce power consumption and extend battery life.
System standby Action When Resume A message box appears 15 seconds before system standby takes effect. This allows you to stop the process and save your work if you have not recently done so.
In Windows 98 and Windowsstandby and suspend are equivalent terms. Traveling with Your Computer Managing Power Consumption To use manual power management You can shut down your computer manually whenever you need to with any of the following three levels of shutdown.
System standby Action Turns off the display and hard disk drive. When Press the blue standby button. Note When the system hibernates as a result of a critically low battery, you will find when resuming that all your data has been saved but that some functions may be disabled.
Using Battery Power Using Battery Power You can operate the HP notebook PC on battery power for extended periods, but you should be prepared to save your work and shut down if the battery is low.
HP (Hewlett-Packard) OMNIBOOK XE3 user manual
Follow the tips in this section to maximize the length of time you can operate on battery power. From the Windows taskbar The System Tray on the Windows taskbar includes a power icon that allows access to more detailed battery status information. The icon looks like a battery when AC is not connected. Place pointer over the power icon to display a percentage of remaining time.
Right-click the power icon to view the options for displaying power information. Turn off the CD player when you are not using it. To connect the modem 1. Check that the telephone line is an analog line, sometimes called a data line.
It should have 2, 3, or 4 wires. You must not use a PBX or digital line. Connect a telephone cord with an RJ plug to the built-in modem or connect to a PC card or external modem. You are now ready to explore the Internet. To go to a specific website, enter the address for example, www.
To disconnect from the Internet You remain connected to the Internet until you shut down the computer, disconnect the phone line from the computer, or give a command to hang up the connection. To disconnect from other ISPs, double-click the connect icon two connected computers next to the clock on the taskbar, and click Disconnect. On the General tab, click Properties, then on the Connections tab click Advanced. You can type AT commands in the space for extra settings.
On the Modems tab, click Properties. You can use the modem and fax software to send and receive faxes on your computer. In QuickLink, click the Fax Viewer tool on the toolbar.
Click Yes to view the fax now. Click the printer icon to print the fax. Making Connections Using the Modem To receive, view, and print faxes With Windowsif you enabled the modem to receive faxes, they are received automatically.
Instant WirelessTM Series. Use this guide to install: WET User Guide Linksys guarantees that every Wireless Ethernet Bridge will be free from physical . View and Download Linksys WET11 user manual online. Wireless-B Ethernet Bridge. WET11 Network Router pdf manual download. View and Download Linksys WET11 – Instant Wireless EN Bridge Network Converter quick start manual online. Quick Start Guide. WET11 – Instant Wireless EN.
Linksys is a registered trademark or trademark of Cisco Systems, Inc. Warranty Information Appendix H: Regulatory Information Appendix I: Infrastructure Mode Figure Ad-Hoc Mode Figure Back Panel Figure Front Panel Figure Connect the Ethernet Network Cable Manusl Connect the Power Adapter Figure Check Connection Figure With a Wireless-B Ethernet Linkss in the garage, and another one or a Wireless Access Point in the house, you’re connected—with no cabling hassle.
Let the Wireless-B Ethernet Bridge from Linksys open up exciting new possibilities for your wireless network. This user guide covers the steps for setting up and using the Wireless-B Ethernet Bridge. Planning your Wireless Network This chapter describes the basics of wireless networking.
Regulatory Information This appendix supplies the regulatory information regarding the Bridge. Contact Information This appendix provides contact information for a variety of Linksys resources, including Technical Support.
Wireless-B Ethernet Bridge Chapter 2: Computers in a WLAN share the same frequency channel and SSID, which is an identification name shared by the wireless devices belonging to the same wireless network.
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Wireless-B Ethernet Bridge With these, and many other, Linksys products, your networking options are limitless. Go to the Linksys website at www. Wireless Bridging Using Two Bridges Wireless-B Ethernet Bridge Chapter 3: Re-establish the connection to eliminate we1t1 error. Wireless-B Ethernet Bridge Chapter 4: Wireless-B Ethernet Bridge 4. The screen shown in Figure displays a list of Wireless-B Ethernet Bridges on your network, along with the status information for each Bridge.
If you have only one Bridge on your network, it will be the only one displayed. Select the Bridge you are currently installing by clicking its name in the Selection mamual. Wireless-B Ethernet Bridge 7.
Linksys WET11 User Manual
The Wireless Settings screen, shown in Figurewill now appear. If you chose Ad-Hoc mode, select the channel at which the network broadcasts its wireless signal. Then click the Next button. Wireless-B Ethernet Bridge or fewer alphanumeric characters. It must match the passphrase of your wireless network and is compatible with Linksys wireless products only.
You will have to enter the WEP key s manually on any non-Linksys wireless products.
If you entered a Passphrase, then you will see the automatically generated WEP key in the Key linkssys field, shown in Figure Wireless-B Ethernet Bridge Chapter 6: After configuration, unplug the power cable from the electrical outlet, and unplug the Ethernet network cable from the PC, hub, or switch. Plug the Ethernet network cable into the RJ port on the Ethernet-ready network device you wish to add to the wireless network. Wireless-B Ethernet Bridge Chapter 7: However, if you would like to change these settings or make more advanced configuration changes, use your web browser and the Wireless-B Ethernet Bridge Web-based Utility.
Make sure this setting is the same for all devices in your wireless network. For added security, Linksys recommends that you change the default SSID linksys to a name of your choice.
Setup – Linksys WET11 Wireless-G Ethernet Bridge
To increase wireless network security, using WEP encryption is strongly recommended. To disable WEP encryption, keep the default, Disable. An acronym for Wired Equivalent Privacy, WEP is an encryption method used to protect your wireless data communications.
The Passphrase function is compatible with Linksys wireless products only.
If you want to communicate with non-Linksys wireless products, you will need to enter your WEP key s manually on the non-Linksys wireless products. The range is from 1 to 11Mbps. The rate of data transmission should be set depending on the speed of your wireless network. Firmware updates are posted at www. All information is read-only.
Read the descriptions below to solve your problems. If you can’t find an answer here, check the Linksys website at www. Common Problems and Solutions 1. Perform the following steps: Yes, wef11 long as the game supports multiple players over a LAN. What is ad-hoc mode? When a wireless network is set to ad-hoc mode, the wireless-equipped computers are configured to communicate directly with each other.
Wireless-B Ethernet Bridge janual. This presents a truly revolutionary opportunity to place convenient high speed wireless capabilities in the hands of users around the globe. What is Spread Spectrum? Spread Spectrum technology is a wideband radio frequency technique developed by the military for use in reliable, secure, mission-critical communications systems.
Wireless Security A Brief Overview Whenever data—in the form of files, e-mails, or messages—is transmitted over your wireless network, it is open to attacks. Wireless networking is inherently risky because it broadcasts information on radio waves. Just like signals from your cellular or cordless phone can be intercepted, signals from your wireless network can also be compromised. There are five common ways linkxys hackers can break into your network and steal your bandwidth as well as your data.
Wireless-B Ethernet Bridge examples below, your implementation and administration of network security measures is the libksys to maximizing wireless security. No preventative measure manuaal guarantee network security but it will make it more difficult for someone to hack into your network.
Often, hackers are looking for an easy target. Making your network less attractive to hackers, by making it harder for them to get in, will make them look elsewhere.
So don’t broadcast the SSID. A default SSID is set on your wireless devices by the factory. Hackers know these defaults and can check these against your network. Change your SSID to something unique and not something related to your company or the networking products you use. Wireless-B Ethernet Bridge PCs unprotected by a firewall router should at least run firewall software, and all PCs should run up-to-date antiviral software. This is overstating WEP’s ability.
Linksys wireless products only. If you are not using a Passphrase, then you can enter one or more WEP keys manually. Then, click the Downloads for this Product button. Windows is the most used operating system in the world and comes with many features that help make networking easier. These features can be accessed through Windows Help and are described in this appendix.
Wireless-B Ethernet Bridge Buffer – A block of memory that temporarily holds data to be worked on later when a device is currently too busy to accept the data. Cable Modem – A device that connects a computer to the cable television network, which in turn connects to the Internet. Encryption – Encoding data to prevent it from being read by unauthorized people.
Ethernet – An IEEE standard network protocol that specifies how data is placed on and retrieved from a common transmission medium. Passphrase – Used much like a password, a passphrase simplifies the WEP encryption process by automatically generating the WEP encryption keys for Linksys products.
Linksys product will be free of defects in materials and workmanship under normal use. Your exclusive remedy and Linksys’s entire liability under this warranty will be for Linksys at its option to repair or replace the product or refund Your purchase price less any rebates. Can’t find information about a product you want to buy on the web? Do you want to know more about networking with Linksys products? Give our advice line a call at: Or fax your request in to: If you experience problems with any Manul product, Page of 51 Go.
Planning Your Wireless Networ Page 10 – Figure Ad-Hoc Mode Page 11 – Chapter 3: Getting to Know the Wireless
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In support of this view of parable interpretation, Blomberg not only sets forth Interpreting the Parables can be read with profit by scholars. but the old confusion between ‘apocalyptic’ and ‘eschatology’ obscures the point. Then follows a review of kingdom of God references in the. Synoptics (though. parable interpretation, Blomberg not only sets forth theoretical considerations but devotes attention to all the major parables, providing brief.
See if you have enough points for this item. An Introduction to the New Testament. An Introduction to the Old Testament. Interpreting the New Testament Text. He Who Gives Life. God’s Word in Human Words. God in New Testament Theology. The Composition of the Pentateuch: Renewing the Documentary Hypothesis. Justification of God, The.
Jesus’ Blood and Righteousness. Recovering the Unity of the Bible. Faithful to the End. Rethinking the Historical Jesus, Volume V. Lukan Authorship of Hebrews. Invitation to the Septuagint. Preaching the Old Testament.
Theological Introduction to the Pentateuch, A. Enthroned on Our Praise: An Old Testament Theology of Worship. Soundings in the Theology of Psalms. God and World in the Old Testament. Evangelical Faith and the Challenge of Historical Criticism. A Theology of John’s Gospel and Letters. Jesus and the Forgiveness of Sins.
Collected Interprfting on Scripture. Jesus, the Temple and the Coming Son of Man. The End of the Law. The Way of Wisdom. Canon and Biblical Interpretation. Prophecy and Hermeneutics Studies in Theological Interpretation. Worship in Ancient Israel.
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Rabbi Looks at Jesus of Nazareth, A. The Theology of the Book of Amos. Matthew for Everyone Part 1. Four Portraits, One Jesus. Love in the Parablfs of John. The Epistle to the Romans. Paul and the Mission of the Church. Rudolf Steiner and the Fifth Gospel.
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History of New Testament Research. When Paul Met Jesus. Jesus Christ Our Lord. A Man Attested by God. Paul and the Gentile Problem. That You May Know. A Theology of Mark’s Gospel. Karl Barth and the Fifth Gospel.
Abingdon New Testament Commentaries: The Witness of Jesus, Paul and John. Christ as the Reality. Hebrew Bible and Ancient Versions. Judgment According to Works in Romans. Jesus’ Sermon on the Mount: Matthew 5, 6 and 7. And When You Pray. Historical Dictionary of Jesus. A Manifesto for Theological Interpretation.
Introduction to Biblical Interpretation. A Handbook of New Testament Exegesis. Jesus and the Gospels. Can We Still Believe the Bible? Christians in an Age of Wealth. A Case for Historic Premillennialism. From Pentecost to Patmos. The Historical Reliability of the Gospels. Perspectives on the Sabbath. Introduction to Biblical Interpretation Workbook. The Historical Reliability of the New Testament. Neither Poverty nor Riches. Do Historical Matters Matter to Faith?
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Interpreting the Parables – Craig L. Blomberg – Google Books
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Continue shopping Checkout Continue shopping. Chi ama i libri sceglie Kobo e inMondadori. Interpreting the Parables by Craig L. Or, get it for Kobo Super Points! The problem is that most Bible readers are unlikely ever to know of most of them. In this substantially new and expanded edition, Craig Blomberg surveys and evaluates contemporary critical approaches to the parables, challenging the prevailing consensus and making his own important new contribution to parable studies.
Within proper definitions and boundaries, the author defends a limited allegorical approach. In support of this view inetrpreting parable interpretation, Blomberg not only sets forth theoretical considerations but devotes attention to all the major parables, providing brief interpretations that highlight the insights to be gained from his distinctive method.
Interpreting the Parables can be read with profit by scholars, blomgerg, pastors and educated laypeople. Ratings and Reviews 0 0 star ratings 0 reviews. Overall rating No ratings yet 0. How to write a great review Do Say what you liked best and least Describe the author’s style Explain the rating you gave Don’t Use rude and profane language Include any personal information Mention spoilers or the book’s price Recap the plot.
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The BBC artist page for Antonio Cesti. Find the best clips, watch programmes, catch up on the news, and read the latest Antonio Cesti interviews. Intorno All’ Idol Mio: From the Opera orontea [Antonio Cesti, Robert Sieving] on *FREE* shipping on qualifying offers. Robert Sieving has become . “Intorno all’idol mio”. “Intorno all’idolmio” by, Antonio Cesti is both ardent and melancholy with lovely bel canto vocal effects. It suits a quiet.
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Intorno all’idol mio (Antonio Cesti)
Performances Popular All Recommended. Playlists Featured All Recommended. Artists Popular All Recommended. Antonio Cesti Died 14 October Last Played on BBC. Read more at Wikipedia.
This entry is from Wikipediathe user-contributed encyclopedia. It may not have been reviewed by professional editors and is licensed under an Attribution-ShareAlike Creative Commons License.
If you find the biography content factually incorrect or highly offensive you can edit this article at Wikipedia. Find out more about our use of this data. Show more Show less. Intorno all’idol mio Composer. Tibrino and Gelone’s duet ‘Pur ti ritrovo alfine’: Filosofia’s Aria ‘Sciolta il crin’ from the prologue of ‘Orontea’. Filosofia’s Aria ‘Sciolta il crin’ from the prologue of ‘Orontea’ Composer.
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Ivan Pavlov was a Russian physiologist which is a bit ironic due to the fact that he was a major influence in the field of psychology specifically in Behaviorism. Ivan Pavlov and his theory of classical conditioning had a profound impact on the Behavioral Theory: Thorndike and the Law of Effect. Behaviorism, also known as behavioral psychology, is a theory of In physiologist Ivan Pavlov’s classic experiments, dogs associated the.
Classical conditioning is a reflexive or automatic type of learning in which a stimulus acquires the capacity to evoke a response that was originally evoked by another stimulus. Contributors Theoty Concepts Resources and References.
Several types of learning exist. The most basic form is associative learning, i.
There are two forms of associative learning: In the early twentieth century, Russian physiologist Ivan Pavlov did Nobel prize-winning work on digestion . Over time, he noticed that his dogs who begin salivation before the meat powder was even presented, ivn it was by the presence of the handler or merely by a clicking noise produced by the device that distributed the meat powder.
Fascinated by this finding, Pavlov paired the meat powder with various stimuli such as the ringing of a bell. After the meat powder and bell auditory stimulus were presented together several times, the bell was used alone.
The bell began as a neutral stimulus i. However, by pairing the bell with the stimulus that did produce the salivation response, the bell was able to acquire the ability to trigger the salivation response.
Pavlov therefore demonstrated how stimulus-response bonds which some consider as the basic building blocks of learning are formed. He dedicated much of the rest of his career further exploring this finding. The bell is a neutral stimulus until the dog learns to associate the bell with food.
Then the bell becomes a conditioned stimulus CS which produces the conditioned response CR of salivation after repeated pairings between the bell and food. InWatson studied Albert, an 11 month old infant child.
The goal of the study was to condition Albert to become afraid of a white behxviorist by pairing the white rat with a very loud, jarring noise UCS. At first, Albert showed no sign of fear when he was presented with rats, but once the rat was repeatedly paired with the loud noise UCSBwhaviorist developed a fear of rats.
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Contributors Ivan Pavlov — John B. Watson — Key Concepts Several types of learning exist. This brand new book contains an up-to-date, inclusive account of a core field of psychology research, with in-depth coverage of operant and classical conditioning paclov, its applications, and current topics including behavioral economics.
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Semantic Scholar extracted view of “Entropic elasticity of lambda-phage DNA.” by C. Bustamante et al. Entropic elasticity of λ-phage DNA. C. Bustamante, J. F. Fingerprint. Bacteriophage lambda. Viral DNA. Elasticity. Least-Squares Analysis. scientific article (publication date: 9 September ).
Tests of spool models for DNA packaging in phage lambda. Experiments are reported which bear on two spool models proposed for packaging the DNA of phage lambda. Both spool models fill an assumed spherical cavity with DNA wrapped in cylindrical or quasi-cylindrical layers composed of adjacent circular turns. In the straight spool model, there are several DNA segments in contact with the capsid; they are concentrated in one half probably the left half of lambda DNA.
We have identified the loci on the DNA which are in contact with the capsid by chemical crosslinking, induced by ultraviolet-irradiation of phage containing 5-bromodeoxyuridine in place of thymine. In an electron microscope experiment, phage are first lysed with EDTA, and then spread in a cytochrome c film by the formamide method.
The disrupted capsid, which has the appearance of a phage ghost, serves as a marker showing where the DNA is crosslinked to the coat. The left end of the DNA is not distinguished from the right end, and so the map of DNA -capsid contacts is folded over on itself.
Contacts are found nearly randomly over the entire map. Density centrifugation in a CsCl gradient separates free DNA from restriction fragments crosslinked to protein. After digestion with proteinase k, the DNA fragments previously crosslinked to protein are identified by size after agarose gel electrophoresis. DNA fragments from all parts of the genome are found. These two experiments show that, if the DNA of each phage is packaged identically, then the curved-spool model is ruled out and the straight spool model is unlikely.
Alternatively, the manner of packaging the DNA may vary from one. An inherent problem in the diagnostic PCR assay is the presence of ill-defined inhibitors of amplification which may cause false-negative results. Addition of an amplifiable fragment of foreign DNA in the PCR to serve as a hybrid internal control HIC would allow for a simple way to identify specimens containing inhibitors.
Two oligonucleotide hybrid primers were synthesized to contain nucleic acid sequences of the Chlamydia pneumoniae 16S rRNA primers in a position flanking two primers that target the sequences of a bp lambda phage DNA segment. Of the 14 previously PCR-negative specimens, 13 were confirmed as true negative; 1 had a negative HIC value, indicating inhibition. The assay was then used with nasopharyngeal specimens from patients with pneumonia.
Twenty-one of 8. Specimens showing inhibitory activity were diluted 1: Changes in the rigidity of the polymetric chain of phage lambda double-strand DNA have been studied by laser correlation spectroscopy.
It was shown that, as the ionic strength increases, the effect of the screening of the hydrodynamic interaction of the links of the polymeric chain specific for polymeric coils arises in a DNA solution.
It is assumed that the screening occurs when the threshold of the overlapping of DNA coils is achieved. The abnormal decrease in the DNA coil size in low ionic strength buffers is not a specific feature of lambda phage DNA only. Investigation of interaction between magnetic silica particles and lambda phage DNA fragment. Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial.
Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them – isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods.
In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.
Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimized method with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureus and Escherichia coli.
General transfer matrix formalism to calculate DNA -protein-drug binding in gene regulation: The transfer matrix methodology is proposed as a systematic tool for the statistical-mechanical description of DNA -protein-drug binding involved in gene regulation. We show that a genetic system of several cis-regulatory modules is calculable using this method, considering explicitly the site-overlapping, competitive, cooperative binding of regulatory proteins, their multilayer assembly and DNA looping.
In the methodological section, the matrix models are solved for the basic types of short- and long-range interactions between DNA -bound proteins, drugs and nucleosomes.
We apply the matrix method to gene regulation at the O R operator of phage lambda. The transfer matrix formalism allowed the description of the lambda -switch at a single-nucleotide resolution, taking into account the effects of a range of inter-protein distances. A large change in the expression pattern may arise in this regime due to anticooperative interactions between DNA -bound RNA polymerases. The applicability of the matrix method to more complex systems is discussed.
Classical blender experiment by Hershey and Chase played a seminal role in illustrating the infectious process of bacteriophage to its host, and showed unequivocally that DNA is responsible for the transmission of heredity. Subsequent works by others have established that interaction between phage particles and bacterial cells is a diffusion-limited process in that, statistically speaking, each collision results in an irreversible infection.
However, such a result is hard to reconcile with the fact that the infection appears to be independent of the density of phage receptors on the bacterial cell membrane. Thus, quantitative experiments showing how a phage finds its receptor and how long does it take would be valuable to this paradoxical view.
Using a shear flow apparatus we study absorption kinetics of lambda phage on E. Coli strain YMEL under different flow conditions, and the results are compared with a simple diffusion model taking into account the off convection and the interaction time tau. Rifampin-stimulated uv resistance of phage lambda on Escherichia coli K The plaque survival elasyicity uv-irradiated phage lambda on excision-proficient E.
This repair is accompanied by little or no clear-plaque mutagenesis. Host cells uv-irradiated and incubated for 30 minutes in growth medium prior to treatment with leasticity show some Weigle-reactivation in addition to the repair stimulated by rifampin. Some clear-plaque Weigle-mutagenesis is also observed in the presence of rifampin: These results suggest that repair of lambda on unirradiated elasticiy in the presence of rifampin is an excision-dependent, error-free phenomenon.
Since initiation of replication of lambda DNA requires RNA polymerase, and since rifampin blocks transcription by that polymerase, the effect of rifampin on the survival of lambda may be to delay phage replication, thereby allowing more time for excision repair to operate. The role of template superhelicity in the initiation of bacteriophage elaaticity DNA replication. The binding of multiple copies of the lambda O protein to the phage replication origin ori lambda initiates the ordered assembly of a series of nucleoprotein structures that form at ori lambda prior to DNA unwinding, priming and DNA synthesis steps.
Since the initiation of lambda DNA replication is known to occur only on supercoiled templates in vivo and in vitro, we examined how the early steps in lambda DNA replication are influenced by superhelical tension.
All initiation complexes formed prior to helicase-mediated DNA -unwinding form with high efficiency on relaxed ori lambda DNA. Nonetheless, the DNA templates in these structures must be negatively supertwisted before they can be replicated. Once DNA helicase unwinding is initiated at ori lambdahowever, later steps in lambda DNA replication proceed efficiently in the absence of superhelical tension. Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes.
Background Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome BAC technology. Such modified genomes have potential uses in understanding viral pathogenesis, gene identification and characterization, and the development of new viral vectors and vaccines. While the BAC mutagenesis procedure is a powerful method to generate HSV-2 recombinants, particularly in the absence of selective marker in eukaryotic culture, the mutagenesis procedure is still difficult and cumbersome.
Results Here we describe the incorporation of a phage lambda recombination system into an allele replacement vector. This strategy enables any DNA fragment containing the phage attL recombination sites to be efficiently inserted into the attR sites of the allele replacement vector using phage lambda clonase.
We also describe how etropic incorporation of EGFP into the allele replacement vector can facilitate the selection of the desired cross-over recombinant BACs when the allele replacement reaction is a viral gene deletion.
Finally, we incorporate the lambda phage recombination sites directly into an HSV-2 BAC vector for direct recombination of gene cassettes using the phage lambda clonase-driven recombination reaction.
Entropic elasticity of λ-phage DNA
Conclusion Together, these phahe to the techniques of HSV BAC mutagenesis will facilitate the construction of recombinant herpes simplex viruses and viral vectors. University of Washington, Seattle and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages.
In all cases, endolysin was detected prior to the appearance of mature, intracellular phage and was detected earlier at 44 C than at 37 C. It was synthesized at a linear rate, as was phageand both syntheses terminated at the same time. Under these conditions, endolysin concentration per induced cell was 2 hpage 2.
Eric Siggia – Google Scholar Citations
Since alterations introduced into the lytic elashicity by temperature, mutation, or both correlate well with the timing and rate of endolysin synthesis, the data elasticcity to support the concept that endolysin determines the kinetics of the process.
However, the accumulation of endolysin during lysis inhibition suggests the need for alternative hypotheses. One hypothesis is that although endolysin action is the key to lysis some preliminary steps are required to release the enzyme so that it may contact its substrate in the cell wall. A second hypothesis is that basically the lytic process involves an alteration in the permeability barrier of the cell and that lytic enzymes such as endolysin have evolved as an auxillary but dispensable mechanism to phsge process.
Physical interactions between bacteriophage and Escherichia coli proteins required for initiation of lambda DNA replication. The phag of initiation of lambda DNA replication requires the assembly of the proper nucleoprotein complex at the origin of replication, ori lambda. The complex is composed of both phage and host-coded proteins. The lambda O initiator protein binds specifically to ori lambda.
The lambda P initiator protein binds to both lambda O and the host-coded dna B helicase, slasticity rise to an ori lambda DNA. The dna K and dna J heat shock proteins have been shown capable of dissociating this complex. The thus freed dna B helicase unwinds the duplex DNA template at the replication fork. In this report, through cross-linking, size chromatography, elxsticity protein affinity chromatography, we document some of the kf interactions occurring at ori lambda.
Our results show that the dna K protein specifically interacts with both lambda O and lambda P, and that the dna J protein specifically interacts with the dna B helicase.
The nun gene product of prophage HK excludes phage lambda infection by blocking the expression of genes downstream from the lambda nut sequence. The Nun protein functions both by competing with lambda N transcription-antitermination protein and by actively inducing transcription termination on the lambda chromosome.
We demonstrate that Nun binds directly to a stem-loop structure within nut RNA, boxB, which is also the target for the N antiterminator. The two proteins show comparable affinities for boxB and they compete with each other. Each protein binds the 5′ strand of the boxB stem and the adjacent loop.
Computational aspects of Worm-Like-Chain interpolation formulas – Enlighten: Publications
The stem does not melt upon the binding of Nun or N, as the 3′ strand remains sensitive to a double-strand-specific RNase. Evidently, although Nun and N bind to the same surface of boxB RNA, their respective complexes interact differently with RNA polymerase, inducing transcription termination or antitermination, respectively.
The generation of a strong antibody response to target antigens is a major goal for vaccine development. Here we describe the display of the human immunodeficiency virus HIV envelope spike protein Env on a virus-like scaffold provided by the lambda phage capsid. Phage vectors, in general, have advantages over mammalian virus vectors due to their genetic tractability, inexpensive production, suitability for scale-up, as well as their physical stability, making them an attractive vaccine platform.
Bypassing bacterial infection in phage display by sequencing DNA released from phage particles. Phage display relies on a bacterial infection step in which the phage particles are replicated to perform multiple affinity selection rounds and to enable the identification of isolated clones by DNA sequencing.
Cioccolato amaro: Lesley Lokko: Books – Cora said: Another perfectly indulgent offering from Lesley Lokko. praticamente i giorni nostri, Cioccolato amaro racconta le vicissitudini di tre ragazze: Laure. Premetto che credo che questo sarà l’ultimo romanzo che leggerò della Lokko, sebbene tutti mi parlino bene de L’estate francese e Cioccolato amaro.
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Bitter Chocolate by Lesley Lokko
Refresh and try again. Open Preview See a Problem? Thanks for telling us about the problem. Return to Book Page. Anneliese never talks about her past amarro not even to her only daughter, Callan. She’s worked hard to leave it all behind her and it’s a time and place to which Anneliese will never return. Or so cooccolato thinks. Paperbackpages. Published June 12th by Orion first published June 7th To see what your friends thought of this book, please sign up.
To ask other readers questions about An Absolute Deceptionplease sign up. Be the first to ask a question about An Absolute Deception. Lists with This Book. This book is not yet featured on Listopia. Apr ciovcolato, Justine Crandles rated it it was ok. Oh how I would trade places right now; Melbourne for Bali… was it only 5 weeks ago that we were lying by the pool in the 32 degree heat?
And here I am today… admittedly sick and at home, but with the heater and my favourite trackie lomko on. Well, that is Melbourne for you, the good and the bad weather wise! It stored a collection of new books printed in English Oh how I would trade places right now; Melbourne for Bali… was it only 5 weeks ago that we were lying by the pool in the 32 degree heat?
I came across An Absolute Deception and it caught my eye immediately as I have read two other titles by the same author, and I admittedly loved the cover illustration.
A lengthy pager, I knew it would take me to the end of my trip. Annelise is an A class fashion designer based in London, yet we know her roots are both German and South African. Callan does not know the identity of her elsley, yet never pushes her mother for the details. Her mother reveals nothing of her past to her coiccolato, has come up with a whole new personality in fact to deceive the world of her upbringing cioccolao the events that led her to leave South Africa and come to Europe.
Lokko jumps around between characters and time periods energetically; it creates a strangely disjointed story that I found difficult to engage with. Apartheid, democracy and corruption are laced through the story and highlight a very intelligent author who feels passionately about these topics, yet at times they detract from the main story line. Knowledge on these areas created far more interesting characters who were motivated and educated, however my ability to bond with them ciococlato.
Jul 29, Melanie rated it it was ok. I loved Lesley Lokko’s first book Sundowners The cioccolat book was ok and progressively its gone downhill since then.
This last book was more skim reading than actual reading overall disappointing. Aug 14, Helen rated it liked it.
Lesley Lokko on Apple Books
I’m really disappointed with this book. I really loved Lesley Lokko’s first thre novels but this one was just a real waste of two days of my life. It did take me cioccplato months to really get stuck in but as I’d paid for it on my kindle I thought I would “solider through”. Shouldn’t have bothered really.
The ending was “wet” and diserved more of a “climax”! Nov 18, Jenny Cooke rated it did not like it. Incontriamo Anneliese Zander de Saint Phalle, una famosissima stilista londinese che si appresta a concludere la propria carriera.
Il salto temporale e continentale non fa altro che rendere pesante la lettura, come anche il continuo mutare dei punti di vista. Non ho trovato nulla di diverso da tanti romanzi che si trovano in circolazione, ma soprattutto, non ho trovato un motivo valido che potesse trattenermi su quelle pagine, mentre continuavano a vorticarmi nel pensiero i diritti del lettore di Daniel Pennac.
Feb 05, Larren rated it liked it. This review has been hidden because it contains spoilers. To view it, click here. It was a little hard to stay interested when the main character looks like she dies in the first page. It turned out to be a better read but inconsistencies drive me crazy. How does the main character become flustered with the name of the woman she meets when she’s pesley been told her name beforehand?
Aug 18, Truida rated it really liked it Shelves: My first book by Lesley Lokko. I enjoyed the way she entroduced al the different characters and jump around in time lrsley well as characters with all of them part of everybody elses lives without them knowing it, coming together in the end. I love the way she writes, a very toned down “struggle” novel.
Easy reading but quite enjoyable. May 08, Lucy Hewitt-gudgin rated it it was ok.
Love Lokko as an author but this book lets her down, took a while to get into, was a little boring in pesley, not her usual page turner. The ending, what happened? It felt like the stories were hurriedly thrown together in the last couple of chapters and without piecing it together, several story lines were completely forgotten about. Lesley Lokko does it again. Complex characters spanning Europe and Africa.
It tells the story of Hannelore who becomes pregnant and is sent away from her lkko in disgrace.
She eventually reinvents herself as Annelise a world famous dress designer. The only negative is at the beginning you can become confused as there are so many characters.
I really enjoyed all her other books but I found this one far too long. It was slightly boring in places and very long windedwhere things could have been explained in a couple of sentences a whole chapter was dedicated to it The ending was a bit of a anti- climax too Nov 22, Antonella Serra rated it really liked it.
Good book but has a weird end, incomplete I hope it comes a sequel Feb 22, Francel Anne Julian rated it really liked it. Dec 23, Linda Clarke rated it liked it. Aug 16, Lindsay Hart rated it really liked it. Another good novel from Lesley lokko. Feb 10, Wendy Smyth rated it liked it. Not her best, decidedly predictable. Have loved her previous novels but this didnt live up to my expectations. Aug 07, Jana rated it it was amazing. I just love Lesley Lokko and how her stories are about different people with lives somehow entwined spanning across decades and the world.
Oct 26, Barbara rated it really liked it. Especially liked the parts relating to Namibia. Wonder if there will be a sequel. Nov 11, Rachel rated it liked it. Not a bad read, a bit disjointed. The ending was disappointing as I felt there was far too much left unanswered. Took a while to get going but was a good read once it did.
Jul 30, Rachel rated it really liked it. Loved this book but feel that quite a few parts of the story were left up in the air. Could easily lead to a sequel? May 02, Kate rated it liked it. Jun 25, Kelly rated it liked it. I was excited to read this novel, but unfortunately it did not live up to other Lokko books, good to read though. Boring only read 20 pages and lost interest. Jun 14, Janine rated it it was ok Shelves: I didn’t enjoy this.
It jumped around and the ending was weak. I also could not understand an adult daughter not questioning her mother about her past and her father. Llinos rated it did not like it Dec 20, Jessica Meddick rated it liked it May 04,
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