El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Instpp When colonies developed into microcalli, the alginate beads were protopoastos. Everything you need to know about selling on Stuvia. Genetic Resourses Conservation and Characterization Division. Potato, Solanum tuberosum L. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. Non-transgenic applications of plant tissue culture in potato. Protoplast-fusion derived Solanum cybrids: Prior to fusion, protoplasts from S.

None of the regenerants had 72 chromosomes, which protoplastoz from the protoplasts fusion of a tetraploid with a diploid genome. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding. These results showed that all hybrids were aneuploids Fig. The origin of this chromosome instability has been studied elsewhere in potato Kumar, Ruiz de Galarreta y E.

Protoplasts were precipitated by centrifugation at rpm during 10 minutes. Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats.


Have you written lots of study guides or notes? Shoots also showed purple-colored meristematic zones. The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes. The effect of initial culture media.

These plants were multiplied in glass culture tubes for further analysis. RAPD and chromosomal analysis of the hybrids are also presented.

Origins of cultivated potatoes and species relationships. Aggregations of more than two protoplasts were also observed. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Universidad Nacional Agraria La Molina.

Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis.

Cultivo y fusion de protoplastos [1990]

Final concentration was protoplasts per millilitre. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

Introduction Potato, Solanum tuberosum L. Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. The other parent, the wild species S.

This can be done via morphological, biochemical, protoplastox and molecular markers Pinto et al. Pfotoplastos regeneration started twelve weeks after calli were transferred to MS medium. Isolation of plant DNA from fresh tissue. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.


The enzyme treatment was carried out overnight with gentle agitation. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont.


Segundo curso Primer curso. Regeneration of isolated protoplasts to plants in Solanum dulcamara. None of the plants had a similar banding pattern in relation to each one of the parents. Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, What students say about Stuvia.

Earn while you study. Plant Cell Rep Preview 1 out of 20 pages. Somatic hybridization in the genus Solanum: Plant Cell Tissue Organ Cult.

Due to that, fused and unfused protoplasts were cultured together. An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking.

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